Molecular mass and isoelectric point calculator for glycosylated proteins

Glycosylations should not be neglected for the correct calculation of the molecular mass, the isoelectric point and the mass-specific UV absorption coefficient. Therefore Prot pi provides a tool to draw glycans as a posttranslational modification of proteins. This short guide deals with how to add two complex-type N-Glycosylation G1 with a sialic acid (N-acetylneuraminic acid) […]

MALDI-TOF-MS analysis of antibody-antigen complexes

Editor: Prof. Dr. Christiane Zaborosch Head of Center for Biochemistry Lecturer in Biochemistry christiane.zaborosch@zhaw.ch  Introduction In the development of new drugs, it is of interest in pharmaceutical research to determine the size of antibody-antigen complexes that have formed, since it is assumed that large complexes are cleared more quickly by the body, and thus the […]