Isoelectric point, mass and retention time of proteins are affected by covalent modifications. This section enables the selection of different predefined modifications as well as the definition of multiple user-defined modifications by selecting the "custom"-option. Just select a modification and click "Add Modification"-button. In the new window, detailed parameters can be defined.
please note: All parameters were calculated assuming all cysteine side chains are reduced to sulfhydryl groups. Please specify disulfide bonds by adding a modification.
In this section you can specify advanced parameters for calculations. If you are not familiar with these parameters, you don't have to worry about it. The default settings are certainly correct.
Some further features.